Anticancer activity of anandamide in human cutaneous melanoma cells.

Author(s): Adinolfi B, Romanini A, Vanni A, Martinotti E, Chicca A, Fogli S, Nieri P

Department of Pharmacy, University of Pisa, Via Bonanno 6, 56100 Pisa, Italy. Electronic address: [email protected]

Subject: Cancer View the study


Cannabinoids are implicated in the control of cell proliferation, but little is known about the role of the endocannabinoid system in human malignant melanoma. This study was aimed at characterizing the in vitro antitumor activity of anandamide (AEA) in A375 melanoma cells. The mRNA expression of genes that code for proteins involved in the metabolism and in the mechanism of AEA action was assessed by RT-PCR. Cell viability was tested using WST-1 assay and the apoptotic cell death was determined by measuring caspase 3/7 activities. A375 cells express high levels of fatty acid amide hydrolase (FAAH), cyclooxygenase (COX)-2, cannabinoid receptor 1 (CB1), transient receptor potential cation channel subfamily V member 1 (TRPV1) and G-protein-coupled receptor 55 (GPR55) genes.

Source: European Journal of Pharmacology (Price: $36)
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